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D2Dx™ - A Single-Step and Washing-Free Immunoassay Technology
D2Dx™ (from diameter to diagnostics) detects target
analytes by measuring the average diameter change of gold nanoparticle (AuNP) probes upon binding with the target analytes. To conduct the assay, simply mix a sample solution with the AuNP probe solution. Following an incubation period of 5-30 min, the binding of the target analytes with the AuNP
probes will cause AuNP probes to clump together. The AuNP clumps (aggregates) are detected by measuring the average particle size of the assay solution using D2Dx-R ASSAY READER. The average particle size of the assay solution is correlated to the target analyte concentration.
Compared to the traditional enzyme-linked immunosorbent assay (ELISA), D2Dx™ is much simpler and faster. Click the following YouTube videos to learn more about the difference between multi-step ELISA and D2Dx™ technique.