Example Site Message: Click to Edit/Replace Text

Hours of Operation: Mon - Fri 8:00am - 8:00pm

D2Dx™ Immunity Test 

   One-Minute Test to Detect Immune Responses and Status in Humans and Animals

About Us

Nano Discovery Inc. is a biotechnology company based in Orlando, Florida, United States. We develop, manufacture and sell a rapid blood test product, D2Dx™ immunity test, to monitor the immune status and health of animals and humans.  D2Dx™ technology is protected by multiple granted patents and pending patent applications.


What is D2Dx™ immunity test?

D2Dx™ immunity test is a rapid blood test that detects immune health and function in humans and animals. The test involves a single step process and the result is obtained in less than one minute using a handheld reader device, CT-100. Published and ongoing research show that the test can detect active immune responses associated with infectious diseases; immunity change and disease risk of animals during periparturient period; probability of pregnancy in reproductive animals; and immune health condition and development of neonatal and young animals. D2Dx™ immunity test can be performed on farm, at the animal side, providing animal health information that enables farm owners make timely management and treatment decisions. D2Dx™ immunity test is also a powerful tool being used for immunology research and for human disease studies. Please read more to discover the broad applications of D2Dx™ immunity test. Read More

Agricultural Animal Immune Health Testing

A balanced immune system is important for the welfare, productivity and reproduction of farm animals. Animals with good immune health are less prone to diseases, healthier and more productive.  By testing the immune status of agricultural animals, owners and veterinary practitioners can identify animals with high risk and underlying health problems earlier for prompt treatment; selecting animals with strong immune system for breeding; and avoid excessive use of antibiotics on healthy and low risk animals. Read More

Human Infectious Disease Research

A recent study by a group of researchers shows that the D2Dx™ immunity test can detect Th1 versus Th2 immune responses in COVID-19 patients. These different immune responses can predict the clinical risk of COVID-19 patients in developing severe symptoms and poor clinical outcomes. Researchers also suggest that the D2Dx™ immunity test can be a valuable tool for COVID-19 vaccine research and development. Read More


The prices listed here are for the United States, Canada and Mexico only. For all other countries and regions, please contact us to receive a price quote.


CT-100 is the device used to perform the D2Dx™ immunity test. One-time purchase item.



Test kit that contains the reagents and consumables to perform 100 tests.



Test kit that contains the reagents and consumables to perform 200 tests.



Test kit that contains the reagents and consumables to perform 500 tests.



December 6, 2021 - A peer-reviewed paper published in Scientific Reports - A 1-minute blood test detects decreased immune function and increased clinical risk in COVID-19 patients. Deb C, et al. Scientific Reports 11 Article number 23491 (2021).

November 27, 2021 - A  peer-reviewed paper published in Applied Animal Science on the correlation between D2Dx™ immunity test score and beef cow fertility - Relationship between beef cow fertility and maternal immunity, Corpron, M. et al. Applied Animal Science, Volume 37, Issue 6, P733-737, December 01, 2021

July 19, 2021- Multiple presentations are made at the 2021 American Society of Animal Science annual meeting on the use of D2Dx™ immunity test for farm animal health research (click below to read abstracts published in Journal of Animal Science)

July 11, 2021 - Multiple presentations are made at the 2021 American Dairy Science Association annual meeting on the use of  D2Dx™ immunity test for dairy animal health research

  • P319:  The relative relationship between type 1/type 2 immunity and fertility in Holstein cows
  • P488: Use of a cow-side immune test to improve prediction of metritis
  • 104: Relationship between serum metabolites and milk fatty acid with periparturient diseases in Pacific Northwest dairy farms

June 25, 2021 - Research preprint posted on Research Square

- A one-minute blood test detects decreased immune function and increased clinical risk in COVID-19 patients

April 15, 2021 - Nano Discovery is a proud sponsor of the 13th Annual Dr. Harvey Rubin Memorial Food Animal Veterinary Medical Conference

Conference to be held virtually on April 25th and 26th, 2021

October 16, 2020 -Channel 6 News, Orlando, Florida - Physicians using Nano Discovery product develop new test for COVID-19

Physicians develop test to predict mild or severe COVID-19 symptoms

October 2, 2020 - New study posted on medRxiv

- A One-Minute Blood Test to Monitor Immune Responses in COVID-19 Patients and Predict Clinical Risk of Developing Moderate to Severe Symptoms

September 16, 2020 - New study posted on Research Square

- A Rapid Blood Test to Monitor the Immune Status Change of Dairy Cows and to Evaluate their Disease Risk during Periparturient Period

June 22, 2020 - Research presented at the American Dairy Science Association (ADSA) 2020 Virtual Annual Meeting

- Using a Rapid Blood Test to Study the Impact of Heat Stress on the Immune Status of First-Time Pregnant Heifers and their Calves

December 6, 2018 - Research published in ACS Infectious Diseases

- A Single-Step Gold Nanoparticle-Blood Serum Interaction Assay Reveals Humoral Immunity Development and Immune Status of Animals from Neonates to Adults


Please use the following form to place order or make further inquiries. We will get back to you as soon as possible.