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D2Dx™ Immunity Test 

   One-Minute Test to Detect Immune Responses and Status in Humans and Animals

About Us

Nano Discovery Inc. is a biotechnology company based in Orlando, Florida, United States. We develop, manufacture and sell a rapid blood test product, D2Dx™ immunity test, to monitor the immune status and health of animals and humans.  D2Dx™ technology is protected by multiple granted patents and pending patent applications. Nano Discovery Inc. is a private company established in 2009.


What is D2Dx™ immunity test?

D2Dx™ immunity test is a rapid blood test that detects type 1/type 2 immune balance and immunity change in human and animal blood samples. The test involves a single step process and the result is obtained in less than one minute using a handheld reader device, CT-100. Published research has shown that the test can detect active immune responses associated with infectious diseases; immune status change in agricultural and food animals such as cattle during pregnancy and periparturient period; immune health development of animals from neonates to adults. The test may be used to guide the management of agricultural animals; improve the diagnosis and treatment of human infectious diseases; and provide a valuable tool for immunology research.

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COVID-19 Immune Response Research

A recent study by a group of researchers shows that the D2Dx™ immunity test can detect different immune responses in COVID-19 patients. The researchers are investigating the potential of using the D2Dx™ immunity test to predict the clinical risk level of COVID-19 patients in developing severe symptoms and poor clinical outcomes based on the different immune responses of the patient.

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Agricultural Animal Immune Health Testing

A balanced immune system is important for the welfare, productivity and reproduction of farm animals. Animals with good immune health are less prone to diseases, healthier and more productive.  By closely monitoring the immune status of agricultural animals, owners can discover animals with underlying health problems for prompt treatment; identify animals with strong immune system for breeding; and avoid excessive use of antibiotics on healthy and low risk animals.

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CT-100 is the device used to perform the D2Dx™ immunity test. One-time purchase item.



Test kit that contains the reagents and consumables to perform 100 D2Dx™ immunity tests.



Test kit that contains the reagents and consumables to perform 200 D2Dx™ immunity tests.



Test kit that contains the reagents and consumables to perform 500 D2Dx™ immunity tests.



October 16, 2020 -Channel 6 News, Orlando, Florida - Physicians using Nano Discovery product develop new test for COVID-19

Physicians develop test to predict mild or severe COVID-19 symptoms

October 2, 2020 - New study posted on medRxiv

- A One-Minute Blood Test to Monitor Immune Responses in COVID-19 Patients and Predict Clinical Risk of Developing Moderate to Severe Symptoms

September 16, 2020 - New study posted on Research Square

- A Rapid Blood Test to Monitor the Immune Status Change of Dairy Cows and to Evaluate their Disease Risk during Periparturient Period

June 22, 2020 - Research presented at the American Dairy Science Association (ADSA) 2020 Virtual Annual Meeting

- Using a Rapid Blood Test to Study the Impact of Heat Stress on the Immune Status of First-Time Pregnant Heifers and their Calves

December 6, 2018 - Research published in ACS Infectious Diseases

- A Single-Step Gold Nanoparticle-Blood Serum Interaction Assay Reveals Humoral Immunity Development and Immune Status of Animals from Neonates to Adults


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