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D2Dx™ Immunity Test 

  One-Minute Blood Test for Animal Immune Health Testing

About Us

Nano Discovery Inc. is a biotechnology company based in Orlando, Florida, United States. We develop, manufacture and sell a rapid blood test product, D2Dx™ immunity test, to detect and monitor the immune status and health of farm and companion animals.  D2Dx™ technology is protected by multiple granted patents and pending patent applications.


D2Dx™ immunity test is a rapid blood test for animal immune health testing.  It is a one-of-its-kind test that allows quick and accurate assessment of an animal's immune status in the farm, at the animal side. Farmers and veterinarians can conduct the test in their own local facilities without the need of shipping samples to laboratories and waiting for days to receive the results. Our product is built upon and supported by years of research and extensive data evidence. We are proud to provide satisfaction guarantee to our clients and customers. READ MORE

Dairy Cows - Better Immunity, Lower Disease Risk and Better Fertility

Dairy cows experience many health challenges during the transition period. Immune health plays a critical role in protecting dairy cows from infectious diseases. Studies show that D2Dx™ immunity test can monitor the immune status change of dairy cows during the periparturient period. The test can identify cows with high immunity and reduced risk of developing metritis post calving.

Getting a dairy cow pregnant on time remains a significant challenge in dairy industry.  Reproduction is closely related to immune health and immune status in the animal. Recent studies found that D2Dx™ immunity test can help identify cows with higher pregnancy probability from cows with lower fertility. The difference between high fertility group vs low fertility group can be as large as 40% (60-75% in high fertility vs 30% in low fertility group).

Calves - Better Immunity, Better Health and Feed Efficiency

D2Dx™ immunity test has been used to monitor the immune status and immunity development in newborn calves, neonatal calves and newly weaned feedlot calves. On dairy calves, the test has been used to monitor immunity transfer from mother cows to calves through colostrum feed. There is a positive correlation between  D2Dx™ immunity test score and calf mortality rate and morbidity length. D2Dx™ immunity test adds an effective tool for dairy calf health management.

Other studies show that the test can detect significantly compromised immune function in feedlot calves caused by transportation, stress and infection. The test may be used in feedlot to reduce the use of antibiotic treatment on feeder calves. Feedlot calves with higher immunity scores were found to have better feed efficiency.

Sheep and Lamb - Better Immunity, Lower Fecal Egg Count (FEC)

D2Dx™ immunity test has been used to monitor the immune status and health in ewes and lambs. Studies have found that ewes with higher D2Dx™ immunity test scores tend to have less risk of parasitic infection (lower FEC) and lower somatic cell count (LogSCC) in milk compared to ewes with lower immunity scores. Lambs that can develop better immunity within the first few weeks following birth also tend to have lower FEC.

D2Dx™ immunity test may also be used to identify mother ewes with better immune health for selective breeding. Researchers found a positive correlation between mother ewes' immunity scores during peri-parturient period and the general health of newborn lambs. Lambs born from mother ewes with higher immunity scores tend to have higher immunity scores and show higher average daily gains in the first 60 days following birth.

Using  D2Dx™ Immunity Test for Feed and Nutrition Plan Management

Feed and nutritional supplements are extremely important for animal health. Animals require different types and levels of nutrition supply at different growth stage. The nutrition needs of farm animals also vary according to seasons and their geological locations. There are many feed, nutrition and supplement products in the market that are formulated to improve the immune health of the animals.

D2Dx™ immunity test provide a simple tool for farm owners, managers and veterinarians to monitor the immune health of entire herd conveniently on farm setting. Based on the results, feed and nutrition plans may be adjusted to achieve the best results. Use D2Dx™ immunity test to make informed and timely management decisions.


The prices listed here are for the United States, Canada and Mexico only. For all other countries and regions, please contact us to receive a price quote.


CT-100 is the device used to perform the D2Dx™ immunity test. One-time purchase item.



Test kit that contains the reagents and consumables to perform 200 tests.



Test kit that contains the reagents and consumables to perform 500 tests.



For larger quantities, please contact us to request quote.



January 31, 2023 - A peer-reviewed paper was just published in Journal of Animal Science, showing the use of D2Dx™ immunity test to study the immune health and immune status change in feedlot calves - "Effect of supplemental Zn concentration and trace mineral source on immune function and associated biomarkers of immune status in weaned beef calves received into feedlot" (a study conducted by researchers from Iowa State University and Zinpro Corporation)

January 16, 2023 - Nano Discovery is happy to announce that Dr. Dari Brown from Dari Brown Consulting LLC has joined Nano Discovery as a consultant. Dr. Dr. Brown has 17 + years experience and expertise within the Ag (Feed and Feed Additive) and pet industries, both domestic and international markets within multiple species (dairy, swine, poultry, beef, dog and cat). Dr. Brown held leadership roles in general management (overseeing Operations, R&D/Innovation, Quality, Procurement, Sales & Marketing), business development, sales, marketing, technical service, R&D (feed, feed additive and pet supplements) within the US and International markets. Read more about Dr. Brown here. Welcome Dr. Brown!

November 11, 2022 - Nano Discovery Inc. CEO gave a presentation on  D2Dx™ immunity test technology in the November NIAMRRE member spotlight webinar series. Read the Member Spotlight Recap from Erica Baker here.

October 11, 2022 - Nano Discovery CEO will give a presentation in the upcoming November NIAMRRE (The National Institute of Antimicrobial Resistance Research and Education) member spotlight webinar series - November 8 @ 12:00 pm - 1:00 pm (CST). Visit here for additional details.

Zoom link: https://iastate.zoom.us/j/97722246659?pwd=dUZRVzNLbk5tblJZbHFVMXJkbG9OZz09

October 6, 2022 - Nano Discovery Inc. will sponsor a free webinar on " Inflammation and Immune Health in Dairy Cattle and Sheep During the Peri-parturient Period", to be held on October 18, 2022. Click here to register for the webinar

September 14, 2022 - A presentation will be made at the American Society of Animal Science Western Section (WSASAS) Annual Conference (September 20-22, 2022) on using D2Dx™ immunity test for feedlot cattle immune health testing

  • An Evaluation of a Nutrient Drench Administered to Calves at Arrival into the Feedlot on Subsequent Health Status and Assessment of Immune Status with a Rapid Immunity Test. Abstract No. PSII-1.

May 2, 2022 - We are proud to announce that Nano Discovery Inc. has joined The National Institute of Antimicrobial Resistance Research and Education (NIAMRRE). At Nano Discovery Inc., we are keen to use our product and technology to help improve the animal health and reduce the use of antibiotics in food and companion animals. We look forward to working with the NIAMRRE community!

April 28, 2022  -  Multiple presentations will be made at the 2022 American Dairy Science Association annual meeting on the use of  D2Dx™ immunity test for dairy animal health research

  • The D2Dx immunity test as a measure of immune health in ewes and lambs. Abstract No. 1416.
  • Use of a rapid immunity test as an early pregnancy diagnosis tool. Abstract No. 2095M.  
  • Assessment of the effects of prepartum anti-inflammatory therapies on type 1/type 2 immunity ratio using a rapid blood test. Abstract No. 2464V.
  • Associations between clinical metritis and type 1/type 2 immunity in postpartum Holstein dairy cows. Abstract No. 2170T. 

April 26, 2022 - A presentation will be made at the 2022 American Society of Animal Science annual meeting on the use of D2Dx™ immunity test to monitor newborn and neonatal dairy calf health

  • A nanotechnology base immunity measurement (D2Dx) compared with total serum protein concentration using neonatal Holstein bull calves. Casper, D. Casper Calf Ranch. Abstract No. PSX-B-9 . American Society of Animal Science Annual Meeting, June 26-30, 2022.

December 6, 2021 - A peer-reviewed paper published in Scientific Reports - A 1-minute blood test detects decreased immune function and increased clinical risk in COVID-19 patients. Deb C, et al. Scientific Reports 11 Article number 23491 (2021).

November 27, 2021 - A  peer-reviewed paper published in Applied Animal Science on the correlation between D2Dx™ immunity test score and beef cow fertility - Relationship between beef cow fertility and maternal immunity, Corpron, M. et al. Applied Animal Science, Volume 37, Issue 6, P733-737, December 01, 2021

July 19, 2021- Multiple presentations are made at the 2021 American Society of Animal Science annual meeting on the use of D2Dx™ immunity test for farm animal health research (click below to read abstracts published in Journal of Animal Science)

July 11, 2021 - Multiple presentations are made at the 2021 American Dairy Science Association annual meeting on the use of  D2Dx™ immunity test for dairy animal health research

  • P319:  The relative relationship between type 1/type 2 immunity and fertility in Holstein cows
  • P488: Use of a cow-side immune test to improve prediction of metritis
  • 104: Relationship between serum metabolites and milk fatty acid with periparturient diseases in Pacific Northwest dairy farms

June 25, 2021 - Research preprint posted on Research Square

- A one-minute blood test detects decreased immune function and increased clinical risk in COVID-19 patients

April 15, 2021 - Nano Discovery is a proud sponsor of the 13th Annual Dr. Harvey Rubin Memorial Food Animal Veterinary Medical Conference

Conference to be held virtually on April 25th and 26th, 2021

October 16, 2020 -Channel 6 News, Orlando, Florida - Physicians using Nano Discovery product develop new test for COVID-19

Physicians develop test to predict mild or severe COVID-19 symptoms

October 2, 2020 - New study posted on medRxiv

- A One-Minute Blood Test to Monitor Immune Responses in COVID-19 Patients and Predict Clinical Risk of Developing Moderate to Severe Symptoms

September 16, 2020 - New study posted on Research Square

- A Rapid Blood Test to Monitor the Immune Status Change of Dairy Cows and to Evaluate their Disease Risk during Periparturient Period

June 22, 2020 - Research presented at the American Dairy Science Association (ADSA) 2020 Virtual Annual Meeting

- Using a Rapid Blood Test to Study the Impact of Heat Stress on the Immune Status of First-Time Pregnant Heifers and their Calves

December 6, 2018 - Research published in ACS Infectious Diseases

- A Single-Step Gold Nanoparticle-Blood Serum Interaction Assay Reveals Humoral Immunity Development and Immune Status of Animals from Neonates to Adults


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